EEG–fMRI of idiopathic and secondarily generalized epilepsies

We used simultaneous EEG and functional MRI (EEG–fMRI) to study generalized spike wave activity (GSW) in idiopathic and secondary generalized epilepsy (SGE). Recent studies have demonstrated thalamic and cortical fMRI signal changes in association with GSW in idiopathic generalized epilepsy (IGE). We report on a large cohort of patients that included both IGE and SGE, and give a functional interpretation of our findings. Forty-six patients with GSW were studied with EEG–fMRI; 30 with IGE and 16 with SGE. GSW-related BOLD signal changes were seen in 25 of 36 individual patients who had GSW during EEG–fMRI. This was seen in thalamus (60%) and symmetrically in frontal cortex (92%), parietal cortex (76%), and posterior cingulate cortex/precuneus (80%). Thalamic BOLD changes were predominantly positive and cortical changes predominantly negative. Group analysis showed a negative BOLD response in the cortex in the IGE group and to a lesser extent a positive response in thalamus. Thalamic activation was consistent with its known role in GSW, and its detection in individual cases with EEG–fMRI may in part be related to the number and duration of GSW epochs recorded. The spatial distribution of the cortical fMRI response to GSW in both IGE and SGE involved areas of association cortex that are most active during conscious rest. Reduction of activity in these regions during GSW is consistent with the clinical manifestation of absence seizures

A preliminary study of air-pollution measurement by active remote-sensing techniques

Air pollutants are identified, and the needs for their measurement from satellites and aircraft are discussed. An assessment is made of the properties of these pollutants and of the normal atmosphere, including interactions with light of various wavelengths and the resulting effects on transmission and scattering of optical signals. The possible methods for active remote measurement are described; the relative performance capabilities of double-ended and single-ended systems are compared qualitatively; and the capabilities of the several single-ended or backscattering techniques are compared quantitatively. The differential-absorption lidar (DIAL) technique is shown to be superior to the other backscattering techniques. The lidar system parameters and their relationships to the environmental factors and the properties of pollutants are examined in detail. A computer program that models both the atmosphere (including pollutants) and the lidar system is described. The performance capabilities of present and future lidar components are assessed, and projections are made of prospective measurement capabilities for future lidar systems. Following a discussion of some important operational factors that affect both the design and measurement capabilities of airborne and satellite-based lidar systems, the extensive analytical results obtained through more than 1000 individual cases analyzed with the aid of the computer program are summarized and discussed. The conclusions are presented. Recommendations are also made for additional studies to investigate cases that could not be explored adequately during this study

A Method To Determine Lake Depth and Water Availability on the North Slope of Alaska with Spaceborne Imaging Radar and Numerical Ice Growth Modelling

Spaceborne synthetic aperture radar (SAR) images and numerical ice growth modelling were used to determine maximum water depth and water availability in two areas of the North Slope in northwestern Alaska. SAR images obtained between September 1991 and May 1992 were used to identify when and how many lakes froze completely to the bottom, and how many lakes did not freeze completely to the bottom. At Barrow, on the coast, 60% of the lakes froze completely to the bottom in mid-January alone, and by the end of winter 77% of the lakes were completely frozen. In contrast, 100 km to the south in the 'B' Lakes region, only 23% of the lakes froze completely, and there was no sudden freezing of many lakes as occurred at Barrow. A physically based, numerical model was used to simulate ice growth on the lakes. The simulated maximum ice thickness is 2.2 m. Consequently, any lake where some part of the ice cover does not freeze to the bottom has some water more than 2.2 m deep. For those lakes where the ice cover had frozen completely at some time in the winter, the simulated ice growth curve provides the ice thickness at the time each lake had frozen completely to the bottom and thus the lake's maximum water depth. At Barrow, 60% of the lakes are between 1.4 and 1.5 m deep, and 23% are more than 2.2 m deep. At the 'B' Lakes, 77% of the lakes are more than 2.2 m deep. Thus, there is a considerable contrast in lake depth and water availability between the Barrow and the 'B' Lakes regions. This method is simple to implement, and the relatively inexpensive SAR data have good spatial and temporal coverage. This method could be used to determine lake depth and water availability on the entire North Slope and in other polar and subpolar areas where shallow lakes are common. On s'est servi d'images prises au radar à antenne latérale synthétique (RALS) spatioporté et d'une modélisation numérique de la formation de la glace pour déterminer la profondeur d'eau maximale et la disponibilité de cette eau dans deux régions du versant Nord dans le nord-est de l'Alaska. Des images RALS obtenues entre septembre 1991 et mai 1992 ont servi à identifier quand et comment un grand nombre de lacs avaient gelé sur toute leur profondeur et comment cela ne s'était pas produit pour bien d'autres. À Barrow, sur la côte, 60 p. cent des lacs avaient déjà gelé sur toute leur profondeur à la mi-janvier et, à la fin de l'hiver, 77 p. cent des lacs avaient complètement gelé. Par contre, à 100 km plus au sud, dans la région des lacs "B", seulement 23 p. cent des lacs avaient complètement gelé, et on n'observait pas l'engel soudain de nombreux lacs comme c'était le cas à Barrow. On s'est servi d'un modèle numérique fondé sur des critères physiques pour simuler la formation de la glace sur les lacs. L'épaisseur maximale de la glace simulée est de 2,2 m. En conséquence, tout lac où une partie du manteau glaciel n'atteint pas le fond a une profondeur supérieure à 2,2 m. Pour les lacs dont le manteau glaciel atteignait le fond à un moment quelconque de l'hiver, la courbe de formation simulée de la glace donne l'épaisseur de la glace au moment où chaque lac a gelé sur toute sa profondeur, et donc, la profondeur maximale de ce lac. À Barrow, 60 p. cent des lacs ont entre 1,4 et 1,5 m de profondeur et 23 p. cent ont plus de 2,2 m de profondeur. Dans la région des lacs "B", 77 p. cent des lacs ont plus de 2,2 m de profondeur. Il y a donc un fort contraste dans la profondeur des lacs et la disponibilité de l'eau entre la région de Barrow et celle des lacs "B". Cette méthode est facile à appliquer et les données RALS - relativement bon marché - offrent une bonne couverture spatiale et temporelle. On pourrait utiliser cette méthode pour déterminer la profondeur des lacs et la disponibilité de l'eau sur tout le versant Nord et dans d'autres zones polaires et subpolaires où se trouve un grand nombre de lacs peu profonds

A case study on practical live event sound exposure monitoring

The recently launched WHO Global Standard for Safe Listening Venues and Events aims to make listening safer and more enjoyable for audiences around the world. Some key questions remain on how to practically monitor sound exposure as well as on how patrons’ hearing may be affected after significant exposure. This paper presents a case study where various sound exposure monitoring systems and methods were trialed in an indoor music venue. The aim of the work was to develop and validate a practical, accurate and repeatable technique to track sound exposure across music venues that can be presented in real-time. Results indicate that this can be achieved with no more than four, and as few as two, sound level monitoring locations alongside fixed calibration measurements and a small number of spot measurements at the mix position during a performance

Design of a global soil moisture initialization procedure for the simple biosphere model

Global soil moisture and land-surface evapotranspiration fields are computed using an analysis scheme based on the Simple Biosphere (SiB) soil-vegetation-atmosphere interaction model. The scheme is driven with observed precipitation, and potential evapotranspiration, where the potential evapotranspiration is computed following the surface air temperature-potential evapotranspiration regression of Thomthwaite (1948). The observed surface air temperature is corrected to reflect potential (zero soil moisture stress) conditions by letting the ratio of actual transpiration to potential transpiration be a function of normalized difference vegetation index (NDVI). Soil moisture, evapotranspiration, and runoff data are generated on a daily basis for a 10-year period, January 1979 through December 1988, using observed precipitation gridded at a 4 deg by 5 deg resolution

Comparison of Lidar Methods for Remote Measurement of Air Pollutants

This paper presents quantitative comparisons of several single-ended lidar techniques for the remote measurement of gaseous pollutants. These techniques are divided into two groups. The first group is based on the measurement of energy scattered directly by the gas of interest. The gaseous scattering processes considered are ordinary fluorescence, resonance fluorescence (also called resonance scattering), Raman scattering, and resonant (or nearly resonant) Raman scattering. The second group is based on the measurement of a characteristic differential absorption produced by the gas of interest at two discrete wavelengths, using energy scattered back toward the receiver by a remote reflector other than the gas of interest. The remote reflector may be intermixed with the gas of interest, as is the case with aerosols and atmospheric gases (principally nitrogen), or they may be fixed reflectors such as terrestrial objects or retroflectors. The detectability of a given material will depend on the magnitude and characteristics of the optical interaction with that material. The main characteristics of interest are the cross section, the response time, and the spectral response of the material relative to both the transmit and receive functions of the lidar. These characteristics and their implications for remote sensing will be reviewed for the four direct scatter processes and for the differential absorption technique. The characteristic behavior of the direct backscatter technique is different from the differential absorption technique with respect to sensitivity, concentration of material, and the effect of range. For these reasons, the direct backscatter processes cannot be compared directly to the differential absorption technique. The two techniques can be compared for specific material and system configurations, however. This paper describes specific lidar system configurations and gives the calculated performance level for these systems in both the direct backscatter and differential absorption modes for a wide variety of pollutant monitoring situations. The results of this comparison of techniques indicate that the differential- absorption lidar technique can provide adequate range and sensitivity for a wide variety of pollution monitoring applications involving a number of interesting pollutant materials. No other single technique appears to provide these capabilities for such a wide range of materials

Mitochondrial genome sequences illuminate maternal lineages of conservation concern in a rare carnivore

<p>Abstract</p> <p>Background</p> <p>Science-based wildlife management relies on genetic information to infer population connectivity and identify conservation units. The most commonly used genetic marker for characterizing animal biodiversity and identifying maternal lineages is the mitochondrial genome. Mitochondrial genotyping figures prominently in conservation and management plans, with much of the attention focused on the non-coding displacement ("D") loop. We used massively parallel multiplexed sequencing to sequence complete mitochondrial genomes from 40 fishers, a threatened carnivore that possesses low mitogenomic diversity. This allowed us to test a key assumption of conservation genetics, specifically, that the D-loop accurately reflects genealogical relationships and variation of the larger mitochondrial genome.</p> <p>Results</p> <p>Overall mitogenomic divergence in fishers is exceedingly low, with 66 segregating sites and an average pairwise distance between genomes of 0.00088 across their aligned length (16,290 bp). Estimates of variation and genealogical relationships from the displacement (<it>D</it>) loop region (299 bp) are contradicted by the complete mitochondrial genome, as well as the protein coding fraction of the mitochondrial genome. The sources of this contradiction trace primarily to the near-absence of mutations marking the D-loop region of one of the most divergent lineages, and secondarily to independent (recurrent) mutations at two nucleotide position in the D-loop amplicon.</p> <p>Conclusions</p> <p>Our study has two important implications. First, inferred genealogical reconstructions based on the fisher D-loop region contradict inferences based on the entire mitogenome to the point that the populations of greatest conservation concern cannot be accurately resolved. Whole-genome analysis identifies Californian haplotypes from the northern-most populations as highly distinctive, with a significant excess of amino acid changes that may be indicative of molecular adaptation; D-loop sequences fail to identify this unique mitochondrial lineage. Second, the impact of recurrent mutation appears most acute in closely related haplotypes, due to the low level of evolutionary signal (unique mutations that mark lineages) relative to evolutionary noise (recurrent, shared mutation in unrelated haplotypes). For wildlife managers, this means that the populations of greatest conservation concern may be at the highest risk of being misidentified by D-loop haplotyping. This message is timely because it highlights the new opportunities for basing conservation decisions on more accurate genetic information.</p